Transcription factors are key molecules in the regulation of gene expression in all organisms. The transcription factor LEAFY COTYLEDON 2 (LEC2), which belongs to the DNA-binding protein family, contains a B3 domain. The transcription factor is involved in the regulation of important plant biological processes such as embryogenesis, somatic embryo formation, seed storage protein synthesis, fatty acid metabolism, and other important biological processes. Recent studies have shown that regulates the formation of lateral roots and influences the embryonic resetting of the parental vernalization state. The orthologs of and their regulatory effects have also been identified in some crops; however, their regulatory mechanism requires further investigation. Here, we summarize the most recent findings concerning the effects of LEC2 on plant growth and seed development. In addition, we discuss the potential molecular mechanisms of the action of the gene during plant development.
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http://dx.doi.org/10.3390/genes12121896 | DOI Listing |
Biotechnol Bioeng
November 2024
Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDCA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Many proteins used in industrial and pharmaceutical applications are typically synthesized within the secretory pathway. While yeast and mammalian cells have been engineered to enhance the production of endomembrane-targeted proteins, similar strategies in plant cells remain underexplored. This study investigates the potential of arabidopsis leafy cotyledon 2 (AtLEC2), a key regulator of seed development, to enhance the production of proteins targeted to the endoplasmic reticulum (ER) in Nicotiana benthamiana leaves.
View Article and Find Full Text PDFPlants (Basel)
September 2024
College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China.
is a marker gene for early somatic embryogenesis. We screened and functionally verified a SERK-interacting protein to gain insights into tree-peony somatic embryogenesis. Using PoSERK as bait, we identified PorbcL (i.
View Article and Find Full Text PDFElife
September 2024
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/ Zhongshan Biological Breeding Laboratory, Agricultural College of Yangzhou University, Yangzhou, China.
As a master regulator of seed development, Leafy Cotyledon 1 (LEC1) promotes chlorophyll (Chl) biosynthesis in , but the mechanism underlying this remains poorly understood. Here, we found that loss of function of , a homolog of rice, leads to chlorophyllous embryo, indicating that plays an opposite role in Chl biosynthesis in rice compared with that in . OsNF-YB7 regulates the expression of a group of genes responsible for Chl biosynthesis and photosynthesis by directly binding to their promoters.
View Article and Find Full Text PDFInt J Mol Sci
August 2024
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, 40-007 Katowice, Poland.
The embryogenic transition of plant somatic cells to produce somatic embryos requires extensive reprogramming of the cell transcriptome. The prominent role of transcription factors (TFs) and miRNAs in controlling somatic embryogenesis (SE) induction in plants was documented. The profiling of expression in the embryogenic culture of Arabidopsis implied the contribution of the miR156 and miR169 to the embryogenic induction.
View Article and Find Full Text PDFPlant Cell Rep
April 2024
Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, Shandong Province, China.
Two peanut LEC1-type genes exhibit partial functional redundancy. AhNFYB10 could complement almost all the defective phenotypes of lec1-2 in terms of embryonic morphology, while AhNF-YB1 could partially affect these phenotypes. LEAFY COTYLEDON1 (LEC1) is a member of the nuclear factor Y (NF-Y) family of transcription factors and has been identified as a key regulator of embryonic development.
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