AI Article Synopsis
- Urinary tract infections (UTIs) are frequent bacterial infections primarily caused by enteric bacteria, and different virulence factors help these bacteria colonize the bladder.
- The study investigated the antibiotic resistance of clinical UTI pathogens using disk diffusion and PCR methods, finding that Gentamicin (GEN) was the most effective antibiotic against certain strains.
- Results indicated that the antibacterial efficacy of antibiotics, particularly GEN, depends on urinary pH levels, suggesting that adjusting urinary pH could improve treatments for multidrug-resistant bacterial infections.
Article Abstract
Urinary tract infections (UTIs) are common bacterial infections caused mainly by enteric bacteria. Numerous virulence factors assist bacteria in the colonization of the bladder. Bacterial efflux pumps also contribute to bacterial communication and to biofilm formation. In this study, the phenotypic and genetic antibiotic resistance of clinical UTI pathogens such as , , and were determined by disk diffusion method and polymerase chain reaction (PCR). Following this, different classes of antibiotics were evaluated for their antibacterial activity at pH 5, 6, 7 and 8 by a microdilution method. Gentamicin (GEN) was the most potent antibacterial agent against strains. The effect of GEN on the relative expression of and genes was evaluated by quantitative PCR. The slightly acidic pH (pH 6) and GEN treatment induced the upregulation of antibiotic resistance and QS activator genes in both strains. Consequently, bacteria had become more susceptible to GEN. It can be concluded that antibiotic activity is pH dependent and so the artificial manipulation of urinary pH can contribute to a more effective therapy of multidrug resistant bacterial infections.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697967 | PMC |
| http://dx.doi.org/10.3390/antibiotics10121431 | DOI Listing |
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