Nitrate and nitrite are key components of the global nitrogen cycle. As such, Nature has evolved proteins as biological supramolecular hosts for the recognition, translocation, and transformation of both nitrate and nitrite. To understand the supramolecular principles that govern these anion-protein interactions, here, we employ a hybrid biophysical and approach to characterize the thermodynamic properties and protein dynamics of NrtA from the cyanobacterium for the recognition of nitrate and nitrite.
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| http://dx.doi.org/10.1039/d1cc05879g | DOI Listing |
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