AI Article Synopsis
- * A novel testing method using a combination of zwitterionic peptides and gold colloids allows for the detection of the main protease (M) of the virus, providing a visual indication through rapid color changes.
- * This new test can detect M in breath samples at very low concentrations (<10 nM) and has been validated with no false positives, making it suitable for portable, easy-to-use applications, including possible integration into face masks.
Article Abstract
The transmission of SARS-CoV-2 coronavirus has led to the COVID-19 pandemic. Nucleic acid testing while specific has limitations for mass surveillance. One alternative is the main protease (M ) due to its functional importance in mediating the viral life cycle. Here, we describe a combination of modular substrate and gold colloids to detect M via visual readout. The strategy involves zwitterionic peptide that carries opposite charges at the C-/N-terminus to exploit the specific recognition by M . Autolytic cleavage releases a positively charged moiety that assembles the nanoparticles with rapid color changes (t<10 min). We determine a limit of detection for M in breath condensate matrices <10 nM. We further assayed ten COVID-negative subjects and found no false-positive result. In the light of simplicity, our test for viral protease is not limited to an equipped laboratory, but also is amenable to integrating as portable point-of-care devices including those on face-coverings.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854333 | PMC |
| http://dx.doi.org/10.1002/anie.202112995 | DOI Listing |
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