Cytisine-like alkaloids from the seeds of Hemsl. et Wils.

Two new cytisine-like alkaloids, hositisines C () and D (), were isolated from the seeds of along with four known compounds, (-)-tinctorine (), -adenosine (), 2'-deoxyadenosine (), and 7, 2', 4'-trihydroxy-5-methoxyisoflavanone (). Their structures were established using extensive spectroscopic techniques (UV, IR, CD, HRESIMS, 1 D and 2 D NMR). In the cytotoxic activity, compounds - and 5-fluorouracil (positive control) displayed inhibitory effects against HepG2 cells, exhibiting IC values of 44.52 ± 7.83 μM, 111.49 ± 12.76 μM, 127.72 ± 18.67 μM, and 16.37 ± 3.82 μM.