Reliability of resting metabolic rate between and within day measurements using the Vyntus CPX system and comparison against predictive formulas.

To detect longitudinal changes of resting metabolic rate (RMR) resulting from the effects of energetic stress, reliable RMR measurements are crucial. The Vyntus CPX is a new automated indirect calorimetry system for which RMR reliability has not been determined. Additionally, its agreement with common predictive RMR formulas is unknown. To determine the within and between-day reliability of RMR measurements using the Vyntus CPX system and its agreement with predictive RMR formulas. Young (31  ±  7 years) healthy participants (n = 26, 12 females, 14 males) completed three measurements of RMR, two consecutive measures on the same day, one the day before/after, all under standardised conditions. Reliability was assessed with pairwise comparisons of between-day at the same time (BDST), within day consecutive measurements (WDCM) and between-day different time (BDDT), for parameters of reliability (mean change (MC), intraclass correlation (ICC) and typical error of measurement (TEM)). Measured RMR values (kcal/day) were compared against predictive values of 4 common formulas. Parameters of reliability (mean, (95% confidence interval)) were: -BDST: MC, 0.2(-2.3-2.7)% (p = 0.67); ICC, 0.92(0.84-0.97); TEM, 4.5(3.5-6.2)%. -WDCM: MC, -2.5(-6.2-1.3)% (p = 0.21); ICC, 0.88(0.74-0.88); TEM, 7.0(5.4-9.8)%. -BDDT: MC, -1.5(-4.8-1.9)% (p = 0.57); ICC, 0.90(0.76-0.95); TEM, 6.1(4.8-8.5)%. RMR (measured/predicted) were: 1.04  ±  0.14 (Nelson, p = 0.13), 1.03  ±  0.10 (Mifflin, p = 0.21), 0.98  ±  0.09 (Harris-benedict, p = 0.30), 0.95  ±  0.11 (Cunningham, p = 0.01), 1.00  ±  0.12 (Cunningham, p = 0.90) and 0.96  ±  0.13 (DXA, p = 0.03). The Vyntus CPX is reliable and measured RMR values agreed with four predictive formulas but are lower than Cunningham and DXA RMR estimates for this population.