AI Article Synopsis
- - The study focused on how photobiomodulation (PBM) affects inflamed dental pulp stem cells (IDPSCs) under two nutritional conditions (5% and 10% fetal bovine serum).
- - Results showed that laser PBM significantly boosted cell proliferation at both nutritional levels after 24 and 48 hours, but gene expression was unexpectedly highest in the non-laser group with 5% FBS.
- - The researchers concluded that while PBM has positive effects, more work is needed to optimize irradiation conditions for better cell proliferation and differentiation.
Article Abstract
The present study aimed to investigate photobiomodulation's (PBM) effect on inflamed dental pulp stem cells (IDPSCs) under different nutritional conditions. Cell proliferation and odontogenic differentiation were evaluated using the MTT assay and real-time quantitative reverse transcription PCR, respectively after laser PBM of cells in 5 or 10% fetal bovine serum (FBS) culture conditions. A significant positive effect of laser irradiation on cell proliferation under both nutritional conditions after 24 and 48 h was observed. gene expression increased in the groups with laser irradiation and 5% FBS. Comparison of gene expression levels in the four groups revealed no statistically significant stimulatory effect. The highest gene expression was observed in the non-laser group with 5% FBS. Further studies are required to obtain an irradiation setup to ideally improve inflamed dental pulp stem cells' proliferation and differentiation.
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| http://dx.doi.org/10.2217/rme-2021-0056 | DOI Listing |
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