AI Article Synopsis
- Upf1, typically studied for its role in mRNA decay, shows a significant association with active genes in the fission yeast Schizosaccharomyces pombe, as indicated by high-throughput ChIP data.
- This association is sensitive to RNase, correlates with RNA polymerase II (Pol II) transcription, and affects mRNA expression levels.
- In Upf1-deficient cells, there are notable changes in Pol II occupancy and an increased sensitivity to transcription elongation inhibitors, suggesting Upf1 may play a critical role in regulating gene transcription and Pol II phosphorylation.
Article Abstract
Although the RNA helicase Upf1 has hitherto been examined mostly in relation to its cytoplasmic role in nonsense mediated mRNA decay (NMD), here we report high-throughput ChIP data indicating genome-wide association of Upf1 with active genes in Schizosaccharomyces pombe. This association is RNase sensitive, correlates with Pol II transcription and mRNA expression levels. Changes in Pol II occupancy were detected in a Upf1 deficient (upf1Δ) strain, prevalently at genes showing a high Upf1 relative to Pol II association in wild-type. Additionally, an increased Ser2 Pol II signal was detected at all highly transcribed genes examined by ChIP-qPCR. Furthermore, upf1Δ cells are hypersensitive to the transcription elongation inhibitor 6-azauracil. A significant proportion of the genes associated with Upf1 in wild-type conditions are also mis-regulated in upf1Δ. These data envisage that by operating on the nascent transcript, Upf1 might influence Pol II phosphorylation and transcription.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754637 | PMC |
| http://dx.doi.org/10.1093/nar/gkab1249 | DOI Listing |
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