AI Article Synopsis
- * A new method has been developed that allows ODT to work with incoherent light sources, like fluorescent emissions, by using two coherent light beams to mimic coherent scattering.
- * This technique captures spatial phase information through variations in fluorescent emission over time, enabling detailed 3D reconstruction of fluorescent objects with consistent resolution, as shown in simulations and experiments.
Article Abstract
Optical diffraction tomography (ODT) is an indispensable tool for studying objects in three dimensions. Until now, ODT has been limited to coherent light because spatial phase information is required to solve the inverse scattering problem. We introduce a method that enables ODT to be applied to imaging incoherent contrast mechanisms such as fluorescent emission. Our strategy mimics the coherent scattering process with two spatially coherent illumination beams. The interferometric illumination pattern encodes spatial phase in temporal variations of the fluorescent emission, thereby allowing incoherent fluorescent emission to mimic the behavior of coherent illumination. The temporal variations permit recovery of the spatial distribution of fluorescent emission with an inverse scattering model. Simulations and experiments demonstrate isotropic resolution in the 3D reconstruction of a fluorescent object.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682970 | PMC |
| http://dx.doi.org/10.1364/optica.400547 | DOI Listing |
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