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Comparison of DNA/RNA yield and integrity between PMAP36-mediated and other bacterial lysis methods. | LitMetric

Comparison of DNA/RNA yield and integrity between PMAP36-mediated and other bacterial lysis methods.

J Microbiol Methods

Department of stem cell and regenerative biotechnology, Konkuk University, Gwangjin-gu, Seoul, South Korea. Electronic address:

Published: February 2022

AI Article Synopsis

  • This study examines various methods for isolating bacterial DNA and RNA, focusing on how cell envelope complexity affects extraction efficiency for different species.
  • Researchers compared bead-beating, bacteriolytic protein, and PMAP36-vortexing methods on four gram-negative and six gram-positive bacteria, noting differences in yield and integrity of the nucleic acids.
  • Findings indicate physical disruption methods generally reduce integrity of isolated DNA and RNA, while PMAP36 was most effective in maintaining quality, suggesting that method selection should consider the target species and specific extraction goals.

Article Abstract

Currently, several methods are available for the isolation of bacterial DNA and RNA. However, the diversity and complexity of cell envelope structures limit their efficiency depending on the target bacterial species. In this study, we compared the differences in yield and integrity of RNA prepared from four gram-negative and six gram-positive bacterial species using bead-beating, bacteriolytic protein, and PMAP36-vortexing methods. Similarly, we also compared the efficiency of DNA extraction from Staphylococcus aureus. Physical disruption of bacterial cells showed versatility in breaking cells against all tested species; however, a decrease in the integrity of isolated DNA and RNA was observed. Among membranolytic proteins, PMAP36 showed the most promising results, in terms of both the yield and integrity of the prepared nucleic acids. Our results show that each method has inherent advantages and disadvantages depending on its application. Therefore, the characteristics of each method and target species should be considered before the extraction of bacterial DNA and RNA.

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Source
http://dx.doi.org/10.1016/j.mimet.2021.106396DOI Listing

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