COVID-19 PCR test performance on samples stored at ambient temperature.

J Virol Methods

Genomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey. Electronic address:

Published: March 2022

AI Article Synopsis

  • The COVID-19 pandemic, originating in Wuhan, led to a massive influx of patient samples, overwhelming diagnostic labs globally.
  • The study aimed to assess how different storage temperatures (ambient temperature vs. +4 °C) affected the accuracy of the real-time PCR test on COVID-19 samples.
  • Findings showed that nasopharyngeal and oropharyngeal samples stored at ambient temperature remained PCR positive for at least six days, suggesting this method is viable for resource-limited settings during the pandemic.

Article Abstract

The WHO-named Coronavirus Disease 2019 (COVID-19) infection had become a pandemic within a short time period since it was detected in Wuhan. The outbreak required the screening of millions of samples daily and overwhelmed diagnostic laboratories worldwide. During this pandemic, the handling of patient specimens according to the universal guidelines was extremely difficult as the WHO, CDC and ECDC required cold chain compliance during transport and storage of the swab samples. The aim of this study was to compare the effects of two different storage conditions on the COVID-19 real-time PCR assay on 30 positive nasopharyngeal and/or oropharyngeal samples stored at both ambient temperature (22 ± 2 °C) and +4 °C. The results revealed that all the samples stored at ambient temperature remain PCR positive for at least six days without any false-negative result. In conclusion, transporting and storing these types of swab samples at ambient temperature for six days under resource-limited conditions during the COVID-19 pandemics are acceptable.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8673954PMC
http://dx.doi.org/10.1016/j.jviromet.2021.114404DOI Listing

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