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Plant systems have been used as biofactories to produce recombinant proteins since 1983. The huge amount of data, collected so far in this framework, suggests that plants display several key advantages over existing traditional platforms when they are intended for therapeutic uses, including safety, scalability, and the speed in obtaining the final product.Here, we describe a method that could be applied for the expression and production of a candidate subunit vaccine in Nicotiana benthamiana plants by transient expression, defining all the protocols starting from plant cultivation to target recombinant protein purification.
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http://dx.doi.org/10.1007/978-1-0716-1892-9_6 | DOI Listing |
Food Chem
March 2025
Foodomics Laboratory, Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Madrid 28049, Spain. Electronic address:
The solid cake from plant-based milk production, rich in lipids and proteins, can be repurposed to recover valuable fractions suitable for food, cosmetics, and pharmaceutical applications. This innovative study explored terpenoid-based solvents as modifiers of supercritical CO extractions to obtain ready-to-use extracts rich in fatty acids, bioactive compounds, and a protein concentrate derived from almond and peanut biorefinery. The natural mixtures of 1:1 menthol:1,8-cineole (ME:EU) and 1:2 camphor:1,8-cineole (CA:EU) used at concentrations of 7.
View Article and Find Full Text PDFNutr Metab Cardiovasc Dis
February 2025
Ad hoc Working Group of the Italian Society of Human Nutrition (SINU), Italy.
In recent decades, an increasing body of research has highlighted significant scientific evidence linking adherence to the Mediterranean diet with a reduced risk of chronic diseases. Simultaneously, concerns about the environmental impact of the food system have intensified, particularly considering projected population growth in the coming years. This work introduces a new graphical representation of the traditional Mediterranean dietary model, developed by a dedicated Working Group of the Italian Society of Human Nutrition (SINU).
View Article and Find Full Text PDFRice (N Y)
March 2025
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, South Korea.
Molecular farming for producing biopharmaceuticals in plants is considered an excellent method to replace some of the production methods currently used, and a significant number of recombinant proteins have already shown the potential to facilitate this. In particular, production of activin A, which has a variety of important biological functions in humans, is limited. The purpose of this study was to develop a safe, stable, and efficient plant-based in vitro production system for activin A, assess its biological activity in cancer cells, and demonstrate its potential for use in cancer research.
View Article and Find Full Text PDFPhysiology (Bethesda)
March 2025
Mailman School of Public Health, Columbia University.
There is a fundamental need to consider plant physiology in relation to human health as it encompasses a number of often overlooked issues, from plant based medicines, to nutrition. The goal here is to provide a historical narrative of plant physiological and biological responses to rising CO and climate variability while addressing current controversies. Finally a "next steps" overview of current links between plants and human health, and crucial, unmet research needs.
View Article and Find Full Text PDFNutrients
February 2025
Kos Generating Health, 45007 Toledo, Spain.
Background/objectives: The global shift towards vegan and vegetarian diets has garnered attention for their ethical, environmental, and potential health benefits. These diets are often rich in phytonutrients and antioxidants, which have been associated with lower levels of inflammatory markers, such as C-reactive protein (CRP) and interleukin-6 (IL-6), suggesting a potential protective effect against systemic inflammation and oxidative stress. However, despite these benefits, concerns remain regarding their impact on neurological health due to the possible deficiencies of critical nutrients such as vitamin B12, DHA, EPA, and iron.
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