AI Article Synopsis
- Macrophages are important cells in our body's immune system that help fight diseases and infections.
- Researchers often use a special method to remove these cells from mice to study how they work.
- This study explains a new way to take out macrophages and then replace them with modified ones to help understand what happens during infections like sepsis.
Article Abstract
Macrophages are key innate immune cells involved in a broad spectrum of physiological and pathological processes. Macrophage depletion with clodronate-liposomes is commonly used to investigate functions of macrophages in mice. Here, we describe a protocol that combines the depletion of resident macrophages with the reconstitution of the mice with differentiated, lentivirus-transduced bone marrow-derived macrophages (BMDMs) in the context of an experimental sepsis model. This experimental strategy is easily adapted to other experimental designs. For complete details on the use and execution of this protocol, please refer to Du et al. (2020).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669096 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101004 | DOI Listing |
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