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Generation of mixed murine organoids to model cellular interactions. | LitMetric
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Generation of mixed murine organoids to model cellular interactions.

Ana Krotenberg Garcia Jacco van Rheenen Saskia Jacoba Elisabeth Suijkerbuijk

STAR Protoc

Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands.

Published: December 2021

Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666359PMC
http://dx.doi.org/10.1016/j.xpro.2021.100997DOI Listing

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