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Identification of an epitope shared by the DNA-binding domain of glucocorticoid receptor and the B chain of insulin.
Proc Natl Acad Sci U S A
April 1989
Department of Microbiology, Columbia University, New York, NY 10032.
A monoclonal antibody (8G11-C6) generated by an auto-anti-idiotypic route and directed to a site near the ligand-binding site of the glucocorticoid receptor also binds to native insulin and the B chain of insulin but not to the A chain of insulin. The glucocorticoid receptor and the B chain of insulin, therefore, share a cross-reacting epitope. Examination of the primary sequences of the two proteins revealed a limited number of regions of identity or close homology.
View Article and Find Full Text PDFRegulation of immunity by anti-idiotypic antibodies.
Vet Immunol Immunopathol
December 1987
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
The immune system is under control by idiotypic-anti-idiotypic regulation. This control can operate according to dose of antigen, age of the individual, route of administration etc. and function as a specific stimulator or inhibitor of immune reactivity against a defined antigen.
View Article and Find Full Text PDFMonoclonal antibodies to adenosine receptor by an auto-anti-idiotypic approach.
J Immunol
October 1987
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Monoclonal antiadenosine receptor antibodies have been raised by an auto-anti-idiotypic approach. BALB/c mice were immunized with adenosine 6-aminocaproyl-bovine serum albumin. Hybridoma cell lines were raised and lines that secreted antibodies that bound to rabbit antiadenosine antibodies were obtained.
View Article and Find Full Text PDFA monoclonal antibody (8G11-C6) that is directed to a region near the ligand-binding site of the glucocorticoid receptor was obtained by an auto-anti-idiotypic route, using a derivative of triamcinolone coupled to thyroglobulin to immunize a mouse. The resulting hybridomas were screened for anti-idiotypic antibody (anti-antisteroid) with Fab fragments of affinity-purified polyclonal rabbit anti-triamcinolone antibody. The anti-idiotypes were then screened for binding to rat cytosol glucocorticoid receptor by a depletion procedure, yielding a clone, 8G11-C6, whose specificity for receptor was verified by sucrose density and Western blot analyses.
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