In molecular cloning, digoxigenin is used as a ligand that can be incorporated into DNA and RNA probes and detected after hybridization with an anti-digoxigenin-antibody enzyme conjugate. Methods to label nucleic acids with digoxigenin and to detect digoxigenin-labeled probes are introduced here.
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Digoxigenin activates autophagy in hepatocellular carcinoma cells by regulating the PI3K/AKT/mTOR pathway.
Cancer Cell Int
December 2024
Department of Liver Disease, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, 518033, China.
Article Synopsis
- Hepatocellular carcinoma (HCC) is a highly aggressive cancer, and while current immunotherapy options have limitations like side effects and low effectiveness, traditional Chinese medicine offers less toxic alternatives.
- In a study analyzing 1,444 compounds, digoxigenin (DIG) was identified as a potent inhibitor of HCC cell growth, showing promising results through various experimental assays.
- DIG appears to work by modulating the PI3K/AKT/mTOR signaling pathway to induce autophagy in HCC cells, with further validation in both lab and animal models supporting its potential as a novel treatment for liver cancer.
Both aerosol and primer dimer breakdown for straightforward genotyping based on an integrated immunochromatographic biosensor.
Talanta
April 2025
College of Life Sciences, Northwest University, Xi'an, 710069, China. Electronic address:
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