AI Article Synopsis
- MALDI imaging mass spectrometry (IMS) is a technique used to visualize the distribution of small proteins, peptides, and other molecules in biological tissues while maintaining accurate tissue structure.
- The study focused on analyzing cryopreserved tissue samples using a modified cryo-slicing method that does not require optical coherence tomography (OCT) and involved a process called Carnoy delipidation.
- An automated matrix spray method was implemented to enhance the analysis of proteins and peptides, addressing some limitations of traditional MALDI-IMS techniques.
Article Abstract
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has emerged as a powerful tool for analyzing the spatial distribution of peptides, small proteins, and other molecules within biological tissues. The obtained signals can be correlated with underlying tissue architecture, without any geometrical distortion, enabling the so-called molecular histology. Here, we analyzed cryopreserved tissue samples employing the MALDI-IMS for proteins and peptides. We used a nonstandard OCT-free cryo-slicing protocol, followed by Carnoy delipidation. Automated matrix spray was utilized to circumvent some of MALDI-IMS technology drawbacks in protein and peptide analysis.
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| http://dx.doi.org/10.1007/978-1-0716-1936-0_14 | DOI Listing |
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