Standardization of Sibt. & Sm. and M. Bieb. Extracts with Carbohydrate Digestive Enzyme Inhibitory and Antioxidant Activities.

species growing in Turkey are used for various medicinal purposes in traditional folk medicine. We aimed to evaluate antidiabetic (α-glucosidase and α-amylase inhibition assays), antiobesity (pancreatic lipase inhibition assay), and antioxidant (ABTS and DPPH radical scavenging activities, ferric reducing activity, metal chelating activity, and phosphomolybdenum assay) activities of the extracts obtained from branches, leaves, and fruits of and . The branch (IC = 67.1 ± 1.7 µg/mL) and leaf ethyl acetate extracts (IC = 83.4 ± 0.8 µg/mL) of exhibited the strongest activity on the α-glucosidase enzyme. Besides that, leaf methanol extract exerted remarkable α-amylase inhibitory activity (IC = 950.1 ± 3.5 µg/mL). Only branch and leaf ethyl acetate extract slightly inhibited pancreatic lipase enzyme with 2963.3 ± 736.4 and 2343 ± 557.8 µg/mL IC values, respectively. The RP-HPLC-DAD analysis results demonstrated that the more active extracts are richer in agathisflavone, amentoflavone, and umbelliferone than extracts. With this study, it is concluded that branch and leaf ethyl acetate extracts may be a new source of α-glucosidase enzyme inhibitory activity and agathisflavone, amentoflavone can be used in the standardization of the extracts.