anticancer and antibacterial potentials of selected medicinal plants and isolation and characterization of a natural compound from .

In the current study, five different plants, , , , , and aqueous extracts were prepared and applied against the anticancer and antibacterial activities. It was observed that extract shows the highest anticancer activity with cell viability of 21.5%. All the five plants extract was also used against the inhibition of where extract shows a promising inhibitory activity of 3.2 cm followed by . Furthermore, was subjected to the process of column chromatography as a result a withanolide was isolated. The fast atom bombardment mass spectrometry (FAB-MS) and high resolution fast atom bombardment (HRFAB-MS) [M + 1] indicated molecular weight at 453 and molecular formula CHO. The UV-Vis. spectrum shows absorbance at 210 nm suggesting the presence of conjugated system, and Fourier-transform infrared spectroscopy (FTIR) was recorded to explore the functional groups. Similarly, 1D and 2D NMR spectroscopy techniques such as H, C NMR, correlation spectroscopy (COSY-45°), heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC) and Nuclear Overhauser effect Spectroscopy (NOESY) techniques was carried out to determine the unknown natural product. The collective data of all these techniques established the structure of the unknown compound and recognized as a withanolide.