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Efficient CRISPR-Cas9 editing of major evolutionary loci in sticklebacks. | LitMetric

Efficient CRISPR-Cas9 editing of major evolutionary loci in sticklebacks.

Evol Ecol Res

Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305-5329, USA.

Published: January 2019

AI Article Synopsis

Article Abstract

Background: Stickleback fish are widely used to study the genetic and ecological basis of phenotypic evolution. Although several major loci have now been identified that contribute to evolutionary differences between wild populations, further study of the phenotypes associated with particular genes and mutations has been limited by the difficulty of generating targeted mutations at precise locations in the stickleback genome.

Approach And Aims: We compared different methods of expressing single-guide RNAs (sgRNAs) and Cas9 activity in fertilized stickleback eggs. We used an easily scored pigmentation gene () to screen for molecular lesions, phenotypic effects, and possible germline transmission of newly induced alleles. We then used the optimized CRISPR methods to target two major evolutionary loci in sticklebacks, and . We hypothesized that coding region mutations in the gene would alter body pigmentation and possibly sex determination, and that mutations in the gene would disrupt the formation of most armor plates, fin rays, spines, teeth, and gill rakers.

Results: Targeted deletions were successfully induced at each target locus by co-injecting one-cell stage stickleback embryos with either mRNA or Cas9 protein, together with sgRNAs designed to protein-coding exons. Founder animals were typically mosaic for multiple mutations, which they transmitted through the germline at overall rates of 21 to 100%. We found that the copy of on the X chromosome () has diverged from the on the Y chromosome (). Predicted loss-of-function mutations in the gene dramatically altered pigmentation in both external skin and internal organ, but the same was not true for mutations. Predicted loss-of-function mutations in either the or genes did not lead to sex reversal or prevent fertility. Homozygous loss-of-function mutations in the gene led to complete loss of armor plates, severe reduction or loss of most soft rays in the dorsal, anal, and caudal fins, and severe reductions in tooth and gill raker number. In contrast, long dorsal and pelvic spines remained intact in mutant animals, suggesting that common co-segregation of plate loss and spine reduction in wild populations is unlikely to be due to pleiotropic effects of mutations.

Conclusion: CRISPR-Cas9 approaches can be used to induce germline mutations in key evolutionary loci in sticklebacks. Targeted coding region mutations confirm an important role for and in skin pigmentation and armor plate reduction, respectively. They also provide new information about the functions of these genes in other body structures.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664273PMC

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