AI Article Synopsis
- Therapeutic oligonucleotides have gained regulatory approval in key regions like the US, EU, and Japan, highlighting their significance in drug development.
- Liquid chromatography techniques are essential for ensuring the purity and quality of these oligonucleotides.
- In a study on ion-pair reversed-phase separation, various factors such as stationary phase pore size, mobile phase additives, and column temperature were analyzed, showing their impact on the separation process and potential optimization strategies.
Article Abstract
Therapeutic oligonucleotides have recently been approved in the United States, the EU, and Japan. Hence, the analysis of oligonucleotides is an important topic in drug development. Liquid chromatographic techniques are commonly used for purity verification and the determination of oligonucleotides. In ion-pair reversed-phase separation, several parameters, such as the pore size of the stationary phase, mobile phase additives, and column temperature, were investigated using three types of oligonucleotides (18, 19, and 20 mer). All of the investigated parameters could influence the separation, and they are expected to be useful for optimizing oligonucleotide separation.
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| http://dx.doi.org/10.2116/analsci.21P224 | DOI Listing |
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