AI Article Synopsis
- A novel extraction method using ISOLUTE PLD+ columns was developed to effectively quantify 20 different psychoactive drugs in postmortem blood samples via liquid chromatography-tandem mass spectrometry.
- This new method resulted in cleaner extracts compared to traditional techniques, with strong validation showing high linearity in calibration curves (r values > 0.991) and acceptable accuracy and precision rates.
- The technique demonstrated solid recovery efficiencies (64.6-96.8%) and stability of analytes under various storage conditions, making it a valuable tool for forensic toxicology.
Article Abstract
A novel sample extraction method using an ISOLUTE PLD+ protein and phospholipid removal column was developed for simultaneous quantification of 20 psychoactive drugs, including antidepressants, antipsychotics, sedative-hypnotics, and amphetamines, in postmortem whole blood samples by liquid chromatography-tandem mass spectrometry. The method showed improvement in extract cleanliness compared with traditional protein precipitation and the QuEChERS extraction method. The method was validated for all analytes; the calibration curves showed good linearity, with r values exceeding 0.991. The intra- and interday accuracies and precisions were 87.6-117.5% and 1.0-18.6%, respectively. The recovery efficiencies were in the range of 64.6-96.8%. Matrix effects were observed in the range of 82.6-116.0%. All analytes were stable under different storage conditions. This method was successfully applied in postmortem forensic sample analysis to quantify psychoactive drugs. The method described in the current study will be useful for forensic toxicological investigations.
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| http://dx.doi.org/10.1016/j.forsciint.2021.111130 | DOI Listing |
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