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| http://dx.doi.org/10.1016/j.ajem.2021.11.043 | DOI Listing |
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Robotic versus manual disinfection of global priority pathogens at COVID-19 dedicated hospitals.
Am J Infect Control
January 2025
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal. Electronic address:
Background: Twelve bacterial families were identified as global priority pathogens by the World Health Organization in 2017, recognizing the greatest threat they pose to human health and the declining antibiotic efficacy. Robotics has emerged as a swift and contactless tool for disinfecting bacterial surface contamination in healthcare facilities, however, head-to-head comparison of disinfection efficacy of robotic versus manual disinfections is limited. This study aimed at comparing how robotic disinfection performs over manual disinfection against the global priority pathogens in the healthcare setting.
View Article and Find Full Text PDFSystematic optimisation of crude buccal swab lysate protocols for use with the ForenSeq™ DNA Signature Prep Kit.
Int J Legal Med
January 2025
Division of Forensic Medicine and Toxicology, Department of Pathology, Faculty of Health Science, University of Cape Town, Cape Town, South Africa.
The ForenSeq™ DNA Signature Prep kit has not been thoroughly tested with crude buccal swab lysates in large-scale population studies using massively parallel sequencing (MPS). Commonly used lysis buffers for swabs intending to undergo direct polymerase chain reaction (PCR) are SwabSolution™ and STR GO! Lysis Buffers, and these have been successfully used to generate population data using capillary electrophoresis (CE) systems. In this study, we investigated the performance and optimisation of SwabSolution™ and STR GO! lysates with the ForenSeq™ DNA Signature Prep workflow and addressed the challenge of failed MPS profiles in initial trials.
View Article and Find Full Text PDFEvaluation of the COVID-19 diagnostic performance of Rapiim SARS-CoV-2-H, the highly sensitive SARS-CoV-2 antigen qualitative test kit.
Diagn Microbiol Infect Dis
January 2025
Disease Control and Prevention Center, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
We evaluated the diagnostic performance of Rapiim SARS-CoV-2-H (Rapiim-H)-a point-of-care qualitative antigen test-using nasopharyngeal swabs (NPS) and saliva samples and compared its results with those from antigen quantification and nucleic acid amplification tests. NPS and saliva were collected from patients with confirmed and suspected coronavirus disease (COVID-19). In total, 142 NPS and saliva samples were collected.
View Article and Find Full Text PDFDisposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2.
Talanta
October 2023
Instituto de Química, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil; Instituto Nacional de Ciência e Tecnologia de Bioanalítica, 13084-971, Campinas, SP, Brazil. Electronic address:
This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protective layer.
View Article and Find Full Text PDFPneumococcal transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of serotype 19F in Indonesia.
Infect Genet Evol
November 2024
Eijkman Research Center for Molecular Biology, The National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia. Electronic address:
Genetic evolution of resistance due to mutations and transposon insertions is the primary cause of antimicrobial resistance in Streptococcus pneumoniae. Resistance to macrolide, tetracycline, and chloramphenicol is caused by the insertion of specific genes that carried by transposon (Tn). This study aims to analyze transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of S.
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