AI Article Synopsis
- - Traditional medicinal plants are rich in bioactive natural products, particularly cysteine-rich antimicrobial peptides (Cys-rich AMPs), which have enhanced stability due to their unique disulfide bond structures.
- - In silico analysis identified 80-100 potential Cys-rich AMPs in flax, red clover, and sesame, while proteomic analysis confirmed the presence of 3-10 actual Cys-rich AMPs per plant species.
- - Antibacterial tests showed negative results for some AMPs, suggesting that a diverse approach is necessary for effective AMP discovery, highlighting the potential of these plants for future research.
Article Abstract
Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80-100 Cys-rich AMPs per species from three edible traditional medicinal plants: (flax), (red clover), and (sesame). Bottom-up proteomic analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species, including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources for further AMP discovery and characterization.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659199 | PMC |
| http://dx.doi.org/10.3390/molecules26237304 | DOI Listing |
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