Background: PD-L1 represents a crucial immune checkpoint molecule in the tumor microenvironment, identified as a key target for cancer immunotherapy. A correlation between and EMT-related genes expression in various human cancers has been suggested.
Methods: By ScreenCell filtration, digital droplet PCR and confocal microscopy analysis, we aimed to investigate the expression of and EMT/invasive genes (, , , ) in circulating tumor cells (CTCs) collected from the blood of non-muscle-invasive bladder cancer (NMIBC) patients, assessing the prognostic value of these biomarkers in the disease. Welchs' test and Mann-Whitney U test, correlation index, Kaplan-Meier, Univariate and Multivariate Cox hazard proportional analysis were used.
Results: Higher , and mRNA levels were found in pT1 compared to pTa NMIBC. As evaluated by Kaplan-Meier and Univariate and Multivariate Cox analysis, enhancement of , and expression reduces the recurrent free survival in NMIBC patients.
Conclusions: High , and mRNAs mark the recurrent-NMIBC patients and by reducing the RFS represent negative prognostic biomarkers in these patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656875 | PMC |
| http://dx.doi.org/10.3390/cancers13235989 | DOI Listing |
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