AI Article Synopsis

  • α-Ketoglutaramate (KGM) is a metabolite of L-glutamine that may serve as a biomarker for liver diseases like hepatic encephalopathy and is also important for cancer research.
  • Methods to synthesize KGM have been ineffective in achieving high yield and purity, limiting its availability for study.
  • This new approach improves KGM production to over 97% purity and 75% yield, allowing for both concentrated aqueous solutions and crystal forms, which can be used for broader applications in biocatalytic technology.

Article Abstract

α-Ketoglutaramate (KGM) is an underexamined metabolite of L-glutamine in the metabolic pathway of glutaminase II of α-ketoglutarate formation. Presumably, KGM may be a biomarker of hepatic encephalopathy and other hyperammonemic diseases. This metabolite is a substrate for the ω-amidase enzyme and is used to determine its activity in the study of the biochemistry of various types of cancer. However, the commercial unavailability of KGM hinders its widespread use. Methods for the preparative synthesis of KGM are known, but they either do not provide the proper yield or proper purity of the target product. In this work, a detailed description of the procedures is given that allows the production of KGM with a purity above 97% and a yield of the target product above 75% using L-amino acid oxidase from as a catalyst of L-glutamine conversion. KGM can be obtained both in the form of a highly concentrated aqueous solution and in the form of crystals of sodium salt. The developed methods can be used both for scaling up the synthesis of KGM and for creating economical biocatalytic technologies for the production of other highly purified preparations.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657959PMC
http://dx.doi.org/10.3390/ijms222312748DOI Listing

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