AI Article Synopsis
- The protocol outlines a method for identifying metabolites in respiratory samples from patients infected with SARS-CoV-2 or H1N1.
- It includes detailed steps for sample collection, metabolite extraction, and the use of ultra-high-pressure liquid chromatography (UHPLC) with high-resolution mass spectrometry (HRMS) for data collection.
- The protocol has been specifically tailored for samples containing SARS-CoV-2, and further details can be found in the study by Maras et al. (2021).
Article Abstract
Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639449 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101051 | DOI Listing |
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