Chemotherapy drugs have limited efficacy in breast cancer due to multidrug resistance generated by cancer cells against anticancer drugs. In this study, we developed a novel derivative, 2, 3, 5, 4'-tetrahydroxystilbene (TG1) by modifying 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG). In-vivo zebrafish embryo tests revealed that TG1 showed low toxicity. The equitoxic combination of DOX or DTX with TG1 in MCF-7/Adr reduced the IC50 of DOX or DTX, and the combination index (CI) showed strong synergistic effects in the 1:3 molar ratio of DTX: TG1 and 1:5 molar ratio of DOX: TG1. Moreover, fluorescence images confirmed the cellular uptake of DOX when combined with TG1 in MCF-7/Adr. Western blotting analysis indicated downregulation of p-glycoprotein (P-gp) after MCF-7/Adr treated with TG1. In conclusion, the combined therapy of DTX or DOX and TG1 increases drug efficacy via suppressing the p-glycoprotein efflux pump. These results suggest that TG1 may have potential use for breast cancer patients, especially those with multidrug resistance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651109 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260533 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Novel SARS-COV2 poly epitope phage-based candidate vaccine and its immunogenicity.
Res Pharm Sci
October 2024
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Background And Purpose: The global emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prompted widespread concern. Bacteriophages have recently gained attention as a cost-effective and stable alternative for vaccine development due to their adjuvant properties. This study aimed to design and validate a poly epitope composed of viral proteins.
View Article and Find Full Text PDFInvestigation of Combined Aging and Mullins Stress Softening of Rubber Nanocomposites.
Polymers (Basel)
November 2024
Center of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlín, Czech Republic.
Article Synopsis
- The study examined how thermal aging, UV exposure, and stress softening impact rubber modified with two types of nanoclays, Cloisite Na and Cloisite 20A, by measuring properties like tensile strength and strain at break before and after aging.
- Results indicated that both tensile strength and strain at break decreased by about 50% after one week of aging, attributed to the breaking of chemical bonds between the rubber and nanoparticles.
- Additionally, while thermal aging increased the modulus of elasticity for different rubber samples, only the unmodified rubber showed a significant decrease in shape retention; overall, nanocomposite properties varied differently under aging and UV conditions.*
Variable orthogonality of serine integrase interactions within the ϕC31 family.
Sci Rep
November 2024
School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, James Parsons Building, Byrom Street, L3 3AF, Liverpool, UK.
Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage DNA into the host genome. Each integrase has its unique pair of attP and attB sites, a feature that allows them to be used as orthogonal tools for genome modification applications. In the presence of a second protein, the Recombination Directionality Factor (RDF), integrase catalyses the reverse excisive reaction, generating new recombination sites, attR and attL.
View Article and Find Full Text PDFWhole-transcriptome analysis reveals the effect of retinoic acid on small intestinal mucosal injury in cage-stressed young laying ducks.
Poult Sci
December 2024
Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address:
Article Synopsis
- Retinoic acid (RA), a derivative of vitamin A, is linked to various biological functions but its effects on intestinal oxidative stress in stressed laying ducks had not been studied before this experiment.
- A study involving 160 laying ducks tested different concentrations of RA over a 17-day period, finding that RA improved intestinal health by increasing villus height and antioxidant capacity while decreasing certain damaging markers.
- Whole-transcriptome analysis revealed extensive changes in gene expression related to multiple signaling pathways and suggested that RA may help mitigate endoplasmic reticulum stress and apoptosis, contributing to better redox homeostasis in the intestines.
Transglutaminase 1: Emerging Functions beyond Skin.
Int J Mol Sci
September 2024
Division of Experimental Medicine, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H3A 0C7, Canada.
Transglutaminase enzymes catalyze Ca- and thiol-dependent posttranslational modifications of glutamine-residues that include esterification, hydrolysis and transamidation, which results in covalent protein-protein crosslinking. Among the eight transglutaminase family members in mammals, transglutaminase 1 (TG1) plays a crucial role in skin barrier formation via crosslinking and insolubilizing proteins in keratinocytes. Despite this established function in skin, novel functions have begun merging in normal tissue homeostasis as well as in pathologies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!