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Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections.
PLoS Negl Trop Dis
January 2025
DeWorm3 Project, Seattle, Washington, United States of America.
Background: Historically, soil-transmitted helminth (STH) control and prevention strategies have relied on mass drug administration efforts targeting preschool and school-aged children. While these efforts have succeeded in reducing morbidity associated with STH infection, recent modeling efforts have suggested that expanding intervention to treatment of the entire community could achieve transmission interruption in some settings. Testing the feasibility of such an approach requires large-scale clinical trials, such as the DeWorm3 cluster randomized trial.
View Article and Find Full Text PDFExtracting Optimal Number of Features for Machine Learning Models in Multilayer IoT Attacks.
Sensors (Basel)
December 2024
School of Engineering, Technology and Design, Canterbury Christ Church University, Canterbury CT1 1QU, UK.
The rapid integration of Internet of Things (IoT) systems in various sectors has escalated security risks due to sophisticated multilayer attacks that compromise multiple security layers and lead to significant data loss, personal information theft, financial losses etc. Existing research on multilayer IoT attacks exhibits gaps in real-world applicability, due to reliance on outdated datasets with a limited focus on adaptive, dynamic approaches to address multilayer vulnerabilities. Additionally, the complete reliance on automated processes without integrating human expertise in feature selection and weighting processes may affect the reliability of detection models.
View Article and Find Full Text PDFCreation of an FeP Schreibersite Density Functional Tight Binding Model for Astrobiological Simulations.
J Phys Chem A
January 2025
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550, United States.
The mineral schreibersite, e.g., FeP, is commonly found in iron-rich meteorites and could have served as an abiotic phosphorus source for prebiotic chemistry.
View Article and Find Full Text PDFAssessment of choroidal vessels in healthy eyes using 3-dimensional vascular maps and a semi-automated deep learning approach.
Sci Rep
January 2025
Department of Ophthalmology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
To assess the choroidal vessels in healthy eyes using a novel three-dimensional (3D) deep learning approach. In this cross-sectional retrospective study, swept-source OCT 6 × 6 mm scans on Plex Elite 9000 device were obtained. Automated segmentation of the choroidal layer was achieved using a deep-learning ResUNet model along with a volumetric smoothing approach.
View Article and Find Full Text PDFLineage Tracing by Light-Sheet Microscopy and Computational Reconstruction.
Methods Mol Biol
January 2025
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece.
Article Synopsis
- Modern live imaging techniques, such as light-sheet fluorescence microscopy (LSFM), allow for detailed lineage tracing of cells, revealing their development and behavior in real time.
- LSFM enables imaging of whole organisms over extended periods while minimizing damage to the specimens, significantly enhancing our understanding of cellular dynamics.
- The chapter outlines how to prepare LSFM datasets and introduces three software platforms (MaMuT, Mastodon, and TrackMate) for tracking cell lineages in a crustacean model, Parhyale hawaiensis, through various tracking methods.
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