Cell-SELEX-based selection of ssDNA aptamers for specifically targeting V600E-mutated melanoma.

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Experiment Teaching Center of Functional Subjects, College of Basic Medicine, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang 110122, Liaoning, China.

Published: December 2021

Malignant melanoma is regarded as the most aggressive form of skin cancer, and is responsible for most death caused by skin cancer. mutations occur in approximately 40-50% of melanomas, with V600E being the most common mutation. Testing for mutations and targeted therapy have markedly improved long-term survival for patients with -mutated melanoma. Here, we report two aptamers, CH1 and CH5 generated by Cell-SELEX, against V600E-mutated human melanoma cells A375. The two aptamers exhibited high affinity to target cells with low dissociation constants () in the nanomolar range. Furthermore, the binding of two aptamers to target cells was independent of incubation temperature, and their molecular targets were demonstrated to be membrane proteins on the cell surface. We also demonstrated that aptamer CH1 was able to bind to metastatic colorectal cancer cells harboring V600E mutation, indicating a relationship between aptamer CH1 and V600E-related metastatic cancer. Owing to the structure stability and high selectivity to V600E-mutated targeting cells, aptamer CH1 holds great potential as a molecular probe for the detection of V600E-mutated metastatic melanoma.

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http://dx.doi.org/10.1039/d1an01579fDOI Listing

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