AI Article Synopsis
- A new coprological LAMP method called Copro-LAMPAc was developed to detect a zoonotic nematode affecting humans and animals, focusing on DNA extraction and primer design.
- This method demonstrated an impressive analytical sensitivity of 100 fg and a specificity of 100% when tested against DNA from hosts and other pathogens.
- When evaluated with canine fecal samples, Copro-LAMPAc proved to be more sensitive than traditional optical microscopy, positioning it as a promising tool for epidemiological studies in areas where the nematode is endemic.
Article Abstract
is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the -1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of for epidemiological studies in endemic areas.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633310 | PMC |
| http://dx.doi.org/10.3389/fvets.2021.770508 | DOI Listing |
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