AI Article Synopsis

  • This study analyzed the DNA sequence of ribosomal DNA from the yeast Saccharomyces cerevisiae, cloned into an E. coli plasmid.
  • The research identified part of the structural gene for 5.8S rRNA, located within a 700-base-pair EcoRI fragment.
  • The findings confirm that the 5.8S RNA segment is situated between the 18S and 28S segments in the 42S rRNA precursor and its encoding DNA.

Article Abstract

Direct DNA sequence analysis of Saccharomyces cerevisiae ribosomal DNA cloned in an Escherichia coli plasmid revealed part of the structural gene for 5.8S rRNA at one end of a 700-base-pair EcoRI fragment. Taken with the previously established EcoRI restriction map of the ribosomal repeat unit, this sequence establishes that the yeast 5.8S RNA segment is located between the 18S and 28S segments in the 42S rRNA precursor and in the DNA which codes for it.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC222247PMC
http://dx.doi.org/10.1128/jb.134.1.306-309.1978DOI Listing

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