This study aimed to investigate the enhancement of iron ion on growth, metabolic pathway, and biohydrogen production performance of biohydrogen producing bacteria HAU-M1. Different concentrations of Fe and Fe were respectively added into fermentation broth of photo-fermentation biohydrogen production (PFHP) from corn stalk. Regular sampling test was used to measure the characteristics of fermentation broth and gas, metabolic pathway, energy conversion efficiency, and kinetic of PFHP. The analysis of experimental data showed that the maximum hydrogen yield of 70.25 mL/g was observed at 2500 μmol/L Fe addition, with an energy conversion efficiency of 5.21%, which was 19.98% higher over no-addition. However, the maximum hydrogen content of 51.41% and the maximum hydrogen production rate of 17.82 mL/h were observed at 2000 μmol/L Fe addition. The experimental results revealed that iron ion played a key role in PFHP, which provided a technical support for improving the performance of PFHP.
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