Promoting the granulation process of aerobic sludge via a sustainable strategy of effluent reflux in view of AHLs-mediated quorum sensing.

Aerobic granular sludge (AGS) has excellent performance in wastewater treatment. However, the formation and mechanism of AGS by effluent reflux are not fully understood in sequential batch reactors (SBRs). In this study, two reactors were constructed, among which R1 was the control group, and the R2 reactor refluxed one-fourth of the supernatant of the effluent to the influent water. In the reactor of R2, the granules had better COD and TN removal efficiencies and resistance to external shocks, and AGS produced more extracellular polymeric substances (EPS). Analysis of microbial community indicated that AHLs-mediated microbes, denitrifying microbes, and EPS producers were enriched. At the same time, the correlation between 3OC6-HSL, C8-HSL, C12-HSL and PN was 0.89*, 0.94** and 0.92* respectively, the possible mechanism of enhanced granulation was mainly the promotion of AHLs by effluent reflux. Therefore, the effluent reflux strategy could be an innovative and sustainable strategy that validates the function of AHLs-mediated QS to accelerate aerobic sludge granulation and maintain its structural stability.