The mechanism underlying phosphatidylserine eversion in renal tubule cells following calcium oxalate-mediated damage remains unclear; therefore, we investigated the effects of TGF-β1/Smad signaling on phosphatidylserine eversion in the renal tubule cell membrane during the early stage of kidney stone development. In a rat model of early stage of calcium oxalate stone formation, phosphatidylserine eversion on the renal tubular cell membrane was detected by flow cytometry, and the expression of TGF-β1 (transforming growth factor-β1), Smad7, and phospholipid scramblase in the renal tubular cell membrane was measured by western blotting. We observed that the TGF-β1/Smad signaling pathway increased phosphatidylserine eversion at the organism level. The results of in vitro studies demonstrated that oxalate exposure to renal tubule cells induced TGF-β1 expression, increasing phospholipid scramblase activity and phosphatidylserine eversion in the renal tubule cell membrane. These results indicate that TGF-β1 stimulates phosphatidylserine eversion by increasing the phospholipid scramblase activity in the renal tubule cell membrane during the early stage of kidney stone development. The results of this study form a basis for further detailed research on the development of therapeutic agents that specifically treat urolithiasis and exert fewer adverse effects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784500 | PMC |
| http://dx.doi.org/10.1007/s00240-021-01292-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Different Degrees of Sulfated Laminaria Polysaccharides Recovered Damaged HK-2 Cells and Inhibited Adhesion of Nano-COM and Nano-COD Crystals.
Bioinorg Chem Appl
January 2024
Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
Purpose: The crystal adhesion caused by the damage of renal tubular epithelial cells (HK-2) is the key to the formation of kidney stones. However, no effective preventive drug has been found. This study aims to explore the recovery effects of four Laminaria polysaccharides (SLPs) with different sulfate (-OSO) contents on damaged HK-2 cells and the difference in the adhesion of damaged cells to nanometer calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) before and after recovery.
View Article and Find Full Text PDFIntegrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1.
J Transl Med
August 2023
Medical Laboratory of Jining Medical University, Jining Medical University, Jining, 272067, Shandong, China.
Background: Bladder cancer is a urological carcinoma with high incidence, among which muscle invasive bladder cancer (MIBC) is a malignant carcinoma with high mortality. There is an urgent need to develop new drugs with low toxicity and high efficiency for MIBC because existing medication has defects, such as high toxicity, poor efficacy, and side effects. Jorunnamycin A (JorA), a natural marine compound, has been found to have a high efficiency anticancer effect, but its anticancer function and mechanism on bladder cancer have not been studied.
View Article and Find Full Text PDF[Regulation of Mitochondria on Platelet Apoptosis and Activation].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
June 2023
Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, Jiangsu Province, China . E-mail:
Objective: To explore the regulation of mitochondria on platelet apoptosis and activation, and the relationship between platelet apoptosis and activation.
Methods: Platelets were isolated from peripheral venous blood of healthy volunteers. Cyclosporin A (CsA), which has a protective effect on the function of platelet mitochondria, BAPTA, which can chelate calcium ions across membranes in platelets, and NAC, an antioxidant that reduces the level of intracellular reactive oxygen species, were selected for coincubation with washed platelets, respectively.
[High expression of P-selectin (CD62P) after platelet activation in vitro inhibits bacterial proliferation and induces apoptosis-like changes].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
September 2022
Life Science College, Southwest Forestry University, Kunming 650224, China. *Corresponding authors, E-mail:
Article Synopsis
- The study aimed to assess how P-selectin (CD62P) on platelets interacts with Staphylococcus aureus and Escherichia coli, focusing on the platelets' ability to inhibit bacterial growth and the mechanisms involved.
- Results showed that co-culturing platelets with Staphylococcus aureus significantly reduced its turbidity, while Escherichia coli experienced a slight decrease, indicating that platelets are more effective against SA.
- Findings suggest that increased CD62P expression on platelets leads to decreased bacterial proliferation and induces apoptotic changes in both bacteria, with a stronger effect observed on Staphylococcus aureus compared to Escherichia coli.
Carboxymethylation of Polysaccharide and Its Repair Effect on Damaged HK-2 Cells.
Oxid Med Cell Longev
August 2022
College of Chemistry and Materials Science, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
Objective: is the best traditional medicine for treating kidney calculi in China. This study is aimed at increasing the carboxyl (-COOH) content of polysaccharide (DSP0) and further increasing its antistone activity.
Methods: DSP0 was carboxymethylated with chloroacetic acid at varying degrees.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!