The Transcriptional Differences of Avian CD4CD8 Double-Positive T Cells and CD8 T Cells From Peripheral Blood of ALV-J Infected Chickens Revealed by Smart-Seq2.

Front Cell Infect Microbiol

National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Published: January 2022

It is well known that chicken CD8 T cell response is vital to clearing viral infections. However, the differences between T cell subsets expressing CD8 receptors in chicken peripheral blood mononuclear cells (PBMCs) have not been compared. Herein, we used Smart-Seq2 scRNA-seq technology to characterize the difference of chicken CD8, CD8 αα, CD8 αβ, CD8, and CD4CD8 T cell subsets from PBMCs of avian leukosis virus subgroup J (ALV-J)-infected chickens. Weighted gene co-expression network analysis (WGCNA) and Trend analysis revealed that genes enriched in the "Cytokine-cytokine receptor interaction" pathway were most highly expressed in the CD8 αα T cell population, especially T cell activation or response-related genes including CD40LG, IL2RA, IL2RB, IL17A, IL1R1, TNFRSF25, and TNFRSF11, suggesting that CD8 αα T cells rather than other CD8 subpopulations were more responsive to ALV-J infections. On the other hand, genes involved in the "FoxO signaling pathway" and "TGF-beta signaling pathway" were most highly expressed in the CD4CD8 (CD8) T cell population and the function of CD4CD8 T cells may play roles in negatively regulating the functions of T cells based on the high expression of CCND1, ROCK1, FOXO1, FOXO3, TNFRSF18, and TNFRSF21. The selected gene expressions in CD8 T cells and CD4CD8 double-positive T cells confirmed by qRT-PCR matched the Smart-Seq2 data, indicating the reliability of the smart-seq results. The high expressions of Granzyme K, Granzyme A, and CCL5 indicated the positive response of CD8 T cells. Conversely, CD4CD8 T cells may have the suppressor activity based on the low expression of activation molecules but high expression of T cell activity suppressor genes. These findings verified the heterogeneity and transcriptional differences of T cells expressing CD8 receptors in chicken PBMCs.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631335PMC
http://dx.doi.org/10.3389/fcimb.2021.747094DOI Listing

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