The basidiomycetous yeast Ustilago shanxiensis CBS 10075, which was isolated from a wilting leaf in China, produces mannosylerythritol lipid (MEL) biosurfactants. Here, we report the draft genome sequence of U. shanxiensis CBS 10075, which was 21.7 Mbp in size, with a GC content of 52.55%, comprising 65 scaffolds.
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http://dx.doi.org/10.1128/MRA.00706-21 | DOI Listing |
Microbiol Resour Announc
December 2021
Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
The basidiomycetous yeast Ustilago shanxiensis CBS 10075, which was isolated from a wilting leaf in China, produces mannosylerythritol lipid (MEL) biosurfactants. Here, we report the draft genome sequence of U. shanxiensis CBS 10075, which was 21.
View Article and Find Full Text PDFStud Mycol
June 2015
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; CBS-KNAW Fungal Biodiversity Centre, Yeast Division, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
The subphylum Ustilaginomycotina (Basidiomycota, Fungi) comprises mainly plant pathogenic fungi (smuts). Some of the lineages possess cultivable unicellular stages that are usually classified as yeast or yeast-like species in a largely artificial taxonomic system which is independent from and largely incompatible with that of the smut fungi. Here we performed phylogenetic analyses based on seven genes including three nuclear ribosomal RNA genes and four protein coding genes to address the molecular phylogeny of the ustilaginomycetous yeast species and their filamentous counterparts.
View Article and Find Full Text PDFJ Biosci Bioeng
May 2008
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5-2, Tsukuba, Ibaraki, Japan.
The search for a novel producer of glycolipid biosurfactants, mannosylerythritol lipids (MELs), was undertaken on the basis of the analysis of ribosomal DNA sequences of yeast strains of the genus Pseudozyma. In the course of the investigation, Pseudozyma siamensis CBS 9960, which is closely related to Pseudozyma shanxiensis, a known MEL-C producer but with a different morphology, was found to accumulate a large amount of glycolipids. On thin layer chromatography, the extracellular glycolipids showed nearly the same spots as those of the MELs produced by P.
View Article and Find Full Text PDFJ Oleo Sci
February 2008
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 5-2 Tsukuba Central, 1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as the sole carbon source. Based on thin layer chromatography, the extracellular glycolipids gave spots corresponding to those of mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From the structural characterization by 1H and 13C NMR, the main product was identified as 4-O-[(4'-mono-O-acetyl-2', 3'-di-O-alka(e)noyl)-beta-D-mannopyranosyl]-D-erythritol, which is a highly hydrophilic derivative of MELs known as MEL-C.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
September 2006
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
Eight yeast strains were isolated from jujube fruit surfaces collected in Shanxi and Shandong Provinces, China. All eight strains produced needle-shaped ascospores under suitable conditions. Three separate groups, representing three novel species in the genus Metschnikowia, were recognized by sequence comparisons of the 26S rDNA D1/D2 domain and internal transcribed spacer (ITS) region.
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