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Porcine oviductal extracellular vesicles facilitate early embryonic development via relief of endoplasmic reticulum stress. | LitMetric

AI Article Synopsis

  • - The success of in vitro embryo production (IVEP) relies on replicating the natural environment of the oviduct, although current methods still do not fully employ this environment.
  • - Extracellular vesicles (EVs) in the oviduct play a crucial role in communication between the mother and embryo, and their absence can lead to poor embryo development in lab conditions.
  • - Research indicates that adding oviductal EVs to parthenogenetically activated (PA) embryos enhances development outcomes by improving blastocyst formation and reducing stress in embryo cells, highlighting their importance for improving IVEP techniques.

Article Abstract

The key to successful in vitro embryo production (IVEP) is to mimic the natural in vivo oviductal microenvironment. Although the chemically defined media in extensive use for the in vitro culture of mammalian embryos is based on the composition of oviductal fluid, the IVEP systems in current use must still bypass the oviduct to produce embryos in vitro. Extracellular vesicles (EVs) in the oviduct are versatile intercellular delivery vehicles for maternal-embryo communication, and a lack of them can be associated with failed early embryonic development under in vitro culture conditions. Herein, we isolated EVs from porcine oviduct fluid and confirmed that oviductal EV supplementation improves the embryonic development of parthenogenetically activated (PA) embryos in terms of blastocyst formation rates and total cell numbers. Our experiments also revealed that a beneficial effect of oviductal EVs on PA embryos was achievable, at least in part, by relieving endoplasmic reticulum stress. These results suggest that the maternal-embryo communication mediated by oviductal EVs benefits early embryonic development. Given the contribution of oviductal EVs to early embryonic development, these findings offer novel insights for the optimization of current IVEP systems.

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Source
http://dx.doi.org/10.1002/cbin.11730DOI Listing

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