Combined effect of CO concentration and low-cost urea repletion/starvation in Chlorella vulgaris for ameliorating growth metrics, total and non-polar lipid accumulation and fatty acid composition.

Different CO concentration such as 0.03, 5, 10 and 15% and low-cost urea repletion/starvation in Chlorella vulgaris on growth, total and non-polar lipid content and fatty acid composition was studied. Chlorella vulgaris grown at 0.03% CO apparently revealed inferior biomass yield 0.55 g/L on 14th day compared to CO supplemented cells. In the case of CO supply, 15% CO has unveiled higher biomass yield at about 1.83 g/L on day 12 whereas biomass yield for 5 and 10% CO supplemented cells was 1.61 and 1.73 g/L, respectively on 12th day of cultivation. The biomass productivity (g) per liter per day was 32 mg in control condition whereas it was 125, 134 and 144 mg/L/d in 5, 10 and 15% CO supplied cells, respectively. Lipid content of the strain grown at control, 5, 10 and 15% CO was 21.2, 22.1, 23.4 and 24.6%, respectively and however, without CO addition in low-cost urea repleted and urea depleted medium grown cells revealed 21.2 and 24.2%, respectively. Interestingly, strain grown at 15% CO supply in urea deplete medium yielded 28.7% lipid and contribution of non-polar lipids in total lipids is 69.7%. Further, the fatty acid composition of the strain grown in 15% CO supply in urea depleted medium showed C16:0, C16:1, C18:1 and C18:3 in the level of 30.12, 9.98, 23.43, and 11.97%, respectively compared to control and urea amended condition.