Organophosphate hydrolase (OphB) gene from sp. was transferred into plants to observe the bioremediation ability and tolerance level of the transgenic plant to organophosphate pesticides contaminants. Gene transfer was observed by PCR of the transgenic plants' genomic DNA. Expression of B gene and protein levels in the transgenic plants was observed by western blot analysis. The transgenic plants were resistant and tolerant to chlorpyrifos (an organophosphate pesticide), as evidenced by a toxicity test, where the transgenic plants produced greater shoot and root biomass than that of wild type plants. The fresh weight of transgenic plants' did not reduced significantly till 400 ppm chlorpyrifos treatment, but fresh weight of wild type plants' significantly reduced by the application of 100 ppm chlorpyrifos. Moreover, in 600 ppm chlorpyrifos liquid culture, transgenic plants' produced 1.34 g biomass from 100 seeds, but wild type plants' produced only 0.24 g biomass from 100 seeds. This study indicates that transgenic plants having B gene increase the tolerance level of organophosphate pesticides.

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http://dx.doi.org/10.1080/03601234.2021.2009731DOI Listing

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