The Metagenomic Analysis of Potential Pathogenic Emerging Bacteria in Fleas.

<b>Background and Objective:</b> At present many pathogenic microbes that cause disease in humans are transmitted through animals. <i>Ctenocephalides felis</i>is specific ectoparasites in cats. Metagenomic research on the digestive tract and body surface of <i>C. felis</i>has been conducted. DNA genomics was extracted from the body surface and digestive tract of <i>C. felis</i>. <b>Materials and Methods:</b> Metagenomic analysis has used the 16S rRNA gene (V3-V4 region). Sequencing was carried out using New Generation Sequencing at the First BASE Laboratory, Singapore. <b>Results:</b> Wolbachia has the most significant bacterial composition in <i>C. felis</i> (94.4%), we were found bacteria with a composition >1% that have never been reported to be associated with <i>C. felis</i>. Also, there were 0.2% of bacteria whose taxonomic status cannot be determined. <b>Conclusion:</b> The results of this study become a vital reference pathogenic bacteria that can be transmitted to humans and animals through <i>C. felis</i>. It is necessary to study the resistance of bacteria isolated from<i>C. felis</i>to antibiotics in the future.