Dysbiosis in salivary bacterial diversity of postpartum females and its association with oral health problems and APOs.

The present study investigates the dysbiosis in salivary bacterial diversity by culture-dependent and independent methods. Culturable aerobic and facultative anaerobic bacterial diversity was studied in saliva collected from 267 postpartum and 54 nonpregnant females by using standard microbiological methods. For unculturable bacterial diversity, DNA from saliva samples of four selected females was sequenced by targeting V4 region of 16S rRNA. In postpartum females, was significantly more prevalent. Its colonization was also seen significant among females having gingivitis ( < 0.01), dental caries ( < 0.01), and in those giving birth to low weight baby. In postpartum group, 65.16% females were culture positive for , 12.73% Gram positive rods, 10.48% , 6.36% , 5.61% species and 2.62% . Isolates showed high biofilm forming ability and antibiotic resistance. Upon analysis of unculturable bacterial diversity, a total of 16 phyla and 156 genera were observed. Alpha diversity was decrease in postpartum female having oral health issues with pre-term low weight birth, compared to females with full term birth. Bray-Curtis dissimilarity was highest between female with dental issues and different pregnancy outcomes. Bacterial diversity and abundance altered among females with different oral health conditions and pregnancy outcomes, and also have pathogenic potential.