AI Article Synopsis
- The study examines how different levels of mechanical stretch affect the senescence (aging) of annulus fibrosus (AF) cells in rat intervertebral discs.
- It was found that a 20% stretch significantly reduced cell growth and increased markers associated with aging, while a 2% stretch had less of an impact.
- The findings suggest that high mechanical stretch triggers aging in AF cells via the RhoA/ROCK signaling pathway, providing insights into cell behavior under mechanical stress.
Article Abstract
Background: Intervertebral disc is responsible for absorbing and transmitting mechanical compression. Under physiological conditions, the peripheral annulus fibrosus (AF) cells are subjected to different magnitudes of transverse mechanical stretch depending on the swelling of the central nucleus pulposus tissue. However, the biological behavior of AF cells under mechanical stretch is not well studied.
Objective: This study was performed to study the effects of mechanical tension on AF cell senescence and the potential signaling transduction pathway.
Methods: Rat AF cells were made to experience different magnitudes of mechanical stretch (2% elongation and 20% elongation for 4 hours every day at 1 Hz) in a 10-day experiment period. The inhibitor RKI-1447 of the Rho-associated coiled-coil-containing protein kinases (ROCK) was added along with culture medium to investigate its role. Cell proliferation, cell cycle, telomerase activity, and expression of senescence markers (p16 and p53) were analyzed.
Results: We found that 20% elongation significantly decreased cell proliferation, promoted G0/G1 cell cycle arrest, decreased telomerase activity, and upregulated mRNA/protein expression of p16 and p53. Moreover, the inhibitor RKI-1447 partly resisted effects of 20% elongation on these parameters of cell senescence.
Conclusion: High mechanical stretch obviously induces AF cell senescence through the RhoA/ROCK pathway. This study provides us a deeper understanding on the AF cell's behavior under mechanical stretch.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626192 | PMC |
| http://dx.doi.org/10.1155/2021/5321121 | DOI Listing |
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