Differences between the Leaf Mycobiome of and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content.

The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop with wild species and using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. , , and are the dominant classes of fungi. The core leaf mycobiome among the three species is formed by , , , and . A network analysis showed that , an important pathogen, is negatively related to six fungal species present in and and absent in . Finally, have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than and . The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.