The identification of pathogens causing infectious diseases is still based on laborious and time-consuming techniques. Therefore, there is an urgent need for the development of novel methods and devices that can considerably reduce detection times, allowing the health professionals to administer the right treatment at the right time. Lateral flow-based systems provide fast, cheap and easy to use alternatives for diagnosis. Herein, we report on a lateral flow approach for specifically detecting bacteria within 6 h.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619029 | PMC |
| http://dx.doi.org/10.3390/diagnostics11112022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Both aerosol and primer dimer breakdown for straightforward genotyping based on an integrated immunochromatographic biosensor.
Talanta
November 2024
College of Life Sciences, Northwest University, Xi'an, 710069, China. Electronic address:
Straightforward genotyping can provide timely diagnostic information for diseases prevention and treatment. Taking advantages of speediness and convenience, although numerous genotyping strategies combined loop-mediated isothermal amplification (LAMP) and lateral flow have been reported to satisfy the demand of point-of-care test, the false positive result caused by aerosol and primer dimer as an innate conflict seriously limits their practical application. In this study, both aerosol and primer dimer as extrinsic and intrinsic inducements respectively are first broken through at one stroke based on an integrated immunochromatographic biosensor.
View Article and Find Full Text PDFLateral Flow-Based Skin Patch for Rapid Detection of Protein Biomarkers in Human Dermal Interstitial Fluid.
ACS Sens
November 2024
Department of Mechanical Engineering, Rice University, Houston, Texas 77005, United States.
Rapid diagnostic tests (RDTs) offer valuable diagnostic information in a quick, easy-to-use and low-cost format. While RDTs are one of the most commonly used tools for in vitro diagnostic testing, they require the collection of a blood sample, which is painful, poses risks of infection and can lead to complications. We introduce a blood-free point-of-care diagnostic test for the rapid detection of protein biomarkers in dermal interstitial fluid (ISF).
View Article and Find Full Text PDFNext-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects.
RSC Adv
October 2024
Department of Pharmacy, Birla Institute of Technology and Science, Pilani Vidya Vihar Pilani 333031 Rajasthan India +91 01596 515 835 +91 9660 456 009.
Article Synopsis
- CRISPR/Cas acts like molecular scissors for precise gene editing using mechanisms like non-homologous end joining (NHEJ) or homology-directed repair (HDR), with various Cas effectors available for different applications.
- SpCas9 is the most studied version, recognized as a breakthrough for correcting genetic mutations, while newer variants like Cas12 and Cas13 are noted for their specific abilities to recognize and cleave target nucleic acids.
- The combination of CRISPR technology, diagnostics, and nanotechnology has led to the development of advanced biosensing systems capable of providing quantitative fluorescent signals for diagnostic purposes.
Point-of-care potentials of lateral flow-based field screening for infections: a literature review.
Biol Methods Protoc
May 2024
Department of Biochemistry, Kampala International University-Western Campus, Bushenyi 41201, Uganda.
Point-of-care (POC) field screening for tools for ( is still lacking due to the requirement for a simple, robust field-applicable test that does not entail specialized laboratory equipment. In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines, this review identifies the methodologies that were retrieved based on our search strategy that have been reported for the diagnosis of infection between 2014 and diagnostics. A search criterion was generated to curate 103 articles, which were reduced in number (to 46), following the screening guidelines of PRISMA.
View Article and Find Full Text PDFDevelopment and evaluation of a lateral flow-based portable optical system for determination of the pregnancy status of dairy cows.
J Dairy Sci
October 2024
Department of Animal Science, Cornell University, Ithaca, NY 14853. Electronic address:
Our objectives were to develop and evaluate an integrated system consisting of a lateral flow immunoassay (LFIA) and an electronic portable imaging device for determination of pregnancy status of cows based on plasma concentrations of pregnancy-specific protein B (PSPB). Experiment 1 was conducted to test the performance of the LFIA for PSPB (PSPB-LFIA), whereas experiment 2 was conducted to evaluate the performance of the integrated system, including both the LFIA and imaging device. The PSPB-LFIA strips were made of nitrocellulose membrane with polystreptavidin, anti-mouse antibody, europium anti-PSPB conjugates, and biotin PSPB.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!