d-galacturonic acid (d-GalUA) is the main constituent of pectin, a complex polysaccharide abundant in several agro-industrial by-products such as sugar beet pulp or citrus peel. During several attempts to valorise d-GalUA by engineering the popular cell factory , it became obvious that d-GalUA is, to a certain degree, converted to l-galactonate (l-GalA) by an endogenous enzymatic activity. The goal of the current work was to clarify the identity of the responsible enzyme(s). A protein homology search identified three NADPH-dependent unspecific aldo-keto reductases in baker's yeast (encoded by , and ) that show sequence similarities to known d-GalUA reductases from filamentous fungi. Characterization of the respective deletion mutants and an in vitro enzyme assay with a Gcy1 overproducing strain verified that Gcy1 is mainly responsible for the detectable reduction of d-GalUA to l-GalA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622349 | PMC |
| http://dx.doi.org/10.3390/jof7110914 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acanthopanax seeds polysaccharide improve alcoholic fatty liver disease through the gut-liver axis.
Int J Biol Macromol
January 2025
College of Agriculture, Yanbian University, Jilin Province, Yanji 133002, China. Electronic address:
Naturally derived polysaccharides regulate gut microbiota structure via the gut-liver axis to ameliorate Alcoholic fatty liver disease (AFLD). Acanthopanax seeds are abundant in polysaccharides; however, whether Acanthopanax seed polysaccharides (ASP) improve AFLD through the gut-liver axis remains unclear. In this study, ASP was extracted using ultrasonic-assisted extraction, followed by structural characterization and monosaccharide composition analysis.
View Article and Find Full Text PDFPreparation, structural property, and antioxidant activities of a novel pectin polysaccharide from the flowers of Linn.
Front Nutr
January 2025
Prefabricated Dish Industry Development Research Institute, Zhejiang Dong Fang Polytechnic, Wenzhou, China.
Introduction: Oxidative stress, triggered by an imbalance between reactive oxygen species (ROS) production and cellular antioxidant defense mechanisms, is implicated in various pathological conditions. Plant-derived polysaccharides have gained significant attention as potential natural antioxidants due to their biocompatibility, biodegradability, and structural versatility.
Methods: This study focuses on the purification, structural characterization, and antioxidant activities of a novel pectin polysaccharide (HFPS) isolated from the flowers of Linn.
Simultaneous determination of naphthalimide-labelled monosaccharides in . Hua. polysaccharides utilizing the HPLC-UV technique.
Anal Methods
January 2025
Key Laboratory of Xin'an Medicine of the Ministry of Education, College of Chinese Medicine, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230038, P. R. China.
4-Amidogen-1,8-naphthalimide (ANA), a novel pre-column derivatization reagent, has been successfully synthesized and utilized for the highly sensitive analysis of monosaccharides. ANA reacts with the reducing carbonyl groups of saccharides, facilitating monosaccharide detection. The resulting monosaccharide derivatives were meticulously investigated using High-Performance Liquid Chromatography (HPLC) coupled with ultraviolet (UV) spectroscopy.
View Article and Find Full Text PDFExtraction, structural characteristics, bioactivities and application of polysaccharides from Acanthopanax senticosus (Rupr. Maxim.) harms: A review.
Int J Biol Macromol
January 2025
State Key Laboratory of Integration and Innovation of Classical formula and Modern Chinese Medicines, National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau. Electronic address:
Acanthopanax senticosus, a celebrated herbal remedy, has been cherished in China for its health-promoting properties for over two millennia. The plant's key bioactive constituent, A. senticosus polysaccharide (ASPS), has recently attracted significant interest due to its exceptional biological activities.
View Article and Find Full Text PDFStructural characterization of an apple polysaccharide and its anti-inflammatory effect through suppressing TLR4/NF-κB signaling.
Int J Biol Macromol
January 2025
Department of Pharmacy, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, PR China. Electronic address:
The current study isolated a homogeneous polysaccharide (AP) with a molecular weight of 7.9 kDa from the pomace of Fuji apples. AP was found to consists of rhamnose, galactose, arabinose, glucose, and galacturonic acid in a ratio of 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!