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Sterol-Targeted Laboratory Evolution Allows the Isolation of Thermotolerant and Respiratory-Competent Clones of the Industrial Yeast Saccharomyces cerevisiae.
Microb Biotechnol
January 2025
Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, Paterna, Valencia, Spain.
Sterol composition plays a crucial role in determining the ability of yeast cells to withstand high temperatures, an essential trait in biotechnology. Using a targeted evolution strategy involving fluconazole (FCNZ), an inhibitor of the sterol biosynthesis pathway, and the immunosuppressant FK506, we aimed to enhance thermotolerance in an industrial baker's yeast population by modifying their sterol composition. This approach yielded six isolates capable of proliferating in liquid YPD with μ values ranging from 0.
View Article and Find Full Text PDFPrenatal Lipopolysaccharide Stimulation Modulates Gastrointestinal Immunity and Oxidative Status in Weaned Pigs.
Am J Physiol Gastrointest Liver Physiol
January 2025
Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA.
Gastrointestinal immunity and antioxidant defenses may be bolstered in young animals through prenatal immune system stimulation (PIS), but this is largely uninvestigated in swine. This study tested the hypothesis that PIS could regulate offspring's gastrointestinal immune response and oxidative stress profile. To this end, a PIS model was utilized in sows, delivering low-dose LPS during the final third of gestation to target the developing immune system.
View Article and Find Full Text PDF4D-Printed Magnetic Responsive Bilayer Hydrogel.
Nanomaterials (Basel)
January 2025
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
Despite its widespread application in targeted drug delivery, soft robotics, and smart screens, magnetic hydrogel still faces challenges from lagging mechanical performance to sluggish response times. In this paper, a methodology of in situ generation of magnetic hydrogel based on 3D printing of poly-N-isopropylacrylamide (PNIPAM) is presented. A temperature-responsive PNIPAM hydrogel was prepared by 3D printing, and FeO magnetic particles were generated in situ within the PNIPAM network to generate the magnetic hydrogel.
View Article and Find Full Text PDFPreparation and Gas-Sensitive Properties of Square-Star-Shaped Leaf-Like BiVO Nanomaterials.
Nanomaterials (Basel)
January 2025
School of Communication and Information Engineering, Xi'an University of Science and Technology, Xi'an 710054, China.
In this study, square-star-shaped leaf-like BiVO nanomaterials were successfully synthesized using a conventional hydrothermal method. The microstructure, elemental composition, and gas-sensing performance of the materials were thoroughly investigated. Morphological analysis revealed that BiVO prepared at different reaction temperatures exhibited square-star-shaped leaf-like structures, with the most regular and dense structures formed at 150 °C, exhibiting a large specific surface area of 2.
View Article and Find Full Text PDFBiodegradable and Stimuli-Responsive Nanomaterials for Targeted Drug Delivery in Autoimmune Diseases.
J Funct Biomater
January 2025
School of Mechanical Engineering, School of Basic Science, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Autoimmune diseases present complex therapeutic challenges due to their chronic nature, systemic impact, and requirement for precise immunomodulation to avoid adverse side effects. Recent advancements in biodegradable and stimuli-responsive nanomaterials have opened new avenues for targeted drug delivery systems capable of addressing these challenges. This review provides a comprehensive analysis of state-of-the-art biodegradable nanocarriers such as polymeric nanoparticles, liposomes, and hydrogels engineered for targeted delivery in autoimmune therapies.
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