AI Article Synopsis
- The study explores using Clostridium acetobutylicum MTCC 11,274 to convert black strap molasses into biobutanol, a type of biofuel.
- Significant fermentation conditions, like initial pH and yeast extract concentration, were identified using experimental design methods such as Plackett-Burman Design and central composite design.
- Under optimal conditions, the maximum butanol production reached 10.27 g/L within 24 hours, highlighting the potential of waste black strap molasses as a sustainable biofuel source.
Article Abstract
Microbial reduction of black strap molasses (BSM) by Clostridium acetobutylicum MTCC 11,274 was performed for the production of biobutanol. The optimum fermentation conditions were predicted using one factor at a time (OFAT) method. The identification of significant parameters was performed using Plackett-Burman Design (PBD). Furthermore the fermentation conditions were optimized using central composite design (CCD). The kinetics of substrate utilization and product formation were investigated. Initial pH, yeast extract concentration (g/L) and total reducing sugar concentration (g/L) were found as significant parameters affecting butanol production using C. acetobutylicum MTCC11274. The maximum butanol production under optimal condition was 10.27 + 0.82 g/L after 24 h. The waste black strap molasses obtained from sugar industry could be used as promising substrate for the production of next generation biofuel.
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| http://dx.doi.org/10.1016/j.biortech.2021.126405 | DOI Listing |
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