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Human platelet lysate enhances small lipid droplet accumulation of human MSCs through MAPK phosphorylation.

Stem Cell Res Ther

December 2024

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.

Background: Human platelet lysate (hPL) has emerged as a promising serum substitute to enhance the self-renewal and multipotency of human mesenchymal stem cells (MSCs). Despite its potential, the specific biological mechanisms by which hPL influences MSC phenotypes remain inadequately understood.

Methods: We investigated the biological signaling activated by hPL in two common types of human MSCs: bone marrow-derived MSCs (BMSCs) and adipose-derived MSCs (ASCs).

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Detection of human salivary stress biomarkers using an easy-to-use array sensor based on fluorescent organic molecules.

Biosens Bioelectron

February 2025

Department of Chemical Sciences, University of Catania, viale A. Doria 6, 95125, Catania, Italy; INSTM Udr of Catania, Viale Andrea Doria 6, 95125, Catania, Italy. Electronic address:

Article Synopsis
  • The human body produces catecholamine neurotransmitters like dopamine, adrenaline, and cortisol during stress, making their levels important for stress management and medical use.
  • A new analytical device has been developed that can simultaneously detect these three molecules in human saliva without needing any pre-treatments.
  • The device uses a sensor array with fluorescent chemical receptors, demonstrating high accuracy and selectivity over a concentration range of 1 pM to 1 mM, marking it as the first point-of-care tool for this type of measurement in saliva.
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We have prepared and characterized two diradicaloid systems and that originated from the oxidation of a 1,7-(4-(2,6-di--butyl)phenol)-substituted aza-BODIPY core. The aza-BODIPY diradicaloids were characterized by a large array of experimental and computational methods. The diamagnetic closed-shell state was postulated as the ground state in solution and a solid-state with the substantial thermal population originating from both open-shell diradical and open-shell triplet states observed at room temperature.

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Fluorescence Labeling of Peptides: Finding the Optimal Protocol for Coupling Various Dyes to ATCUN-like Structures.

ACS Org Inorg Au

October 2024

Department of Chemistry and Molecular Biology, Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Medicinaregatan 7B, Gothenburg 413 90, Sweden.

Labeling of peptides and proteins with fluorescent dyes is a key step in functionalizing these structures for a wide array of biological assays. However, coupling strategies of such dyes have not been optimized for the most common compounds, while this step is typically the most precious and costly of the whole synthesis. We searched for the best conditions for attachment of the most widely used fluorescent dyes such as 6-carboxyfluorescein, Rhodamine B, and BODIPY-FL to peptides, where amino terminal Cu(II) and Ni(II) binding site (ATCUN) peptides were used as a model system.

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In controlled supramolecular polymerization, planar π-conjugated scaffolds are commonly used to predictably regulate stacking interactions, with various assembly pathways arising from competing interactions involving side groups. However, the extent to which the nature of the chromophore itself (planar non-planar) affects pathway complexity requires clarification. To address this question, we herein designed a new BOPHY dye 2, where two oppositely oriented BF groups induce a disruption of planarity, and compared its supramolecular polymerization in non-polar media with that of a previously reported planar BODIPY 1 bearing identical substituents.

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