The peripheral blood leukocytes in chronic myeloid leukaemia (CML) patients and healthy donors are separated on ficoll-verografin one-step gradients with 1.077 g/ml density. It is shown that 95-98% of donor granulocytes had the density above 1.077 g/ml. Granulocytes of CML patients consisted of two populations having the density above and below 1.077 g/ml (high density granulocytes-HDG, low density granulocytes-LDG). The electrophoretic mobility (EPM) of LDG is 8-34% higher than that of HDG. As a result of EPM definition of granulocytes affected by hyaluronidase, neuraminidase and RNase it is shown that the high EPM of LDG is due to an increase in the density of sialic acids on their surface.
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